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  • Agarose Gel Electrophoresis

  • Cell Sorting

  • Culturing of Procyclic Trypanasoma brucei cells

  • Culturing of Yeast for Plasmid Isolation

  • Electron microscopy sample preparation

  • Expression and Purification of GST-tagged Proteins

  • Immunofluorescence Microscopy

  • Immunofluorescence Staining

  • Immunoprecipitation

  • Isolation of genomic DNA from Trypanosoma brucei cells

  • Isolation of Plasmids through Mini-prep Protocol

  • Isolation of RNA from Trypanosoma brucei cells

  • Live Cell Imaging

  • Preparation of Competent E. coli Cells

  • Purification of DNA from Agarose Gels

  • Purification of PCR Products

  • SDS-Poly Acrylamide Gel Electrophoresis

  • Silver Staining of Gels

  • Staining of SDS-PAGE Gels with Coomassie Blue Stain

  • Transfection of procyclic Trypanasoma brucei Cells

  • Transformation of E. coli by Heat Shock Method

  • Use of Vacuum Pump

  • Western Blotting (Immunoblotting)