LAM SIEW HONG

LAM SIEW HONG

LAM SIEW HONG

Associate Professor and Assistant Head of Department

Contact Information:

Department of Biological Sciences
National University of Singapore
14 Science Drive 4
Singapore 117543

6516 7379
6779 2486
dbslsh@nus.edu.sg

Academic Qualifications

Ph.D. NUS

Honors & Awards

NUS Faculty of Science Teaching Excellence Award (FTEA) 2012 Honor Roll (AY2009-2011)
NUS Annual Teaching Excellence Award (ATEA) AY2010-2011
NUS Faculty of Science Teaching Excellence Award (FTEA) AY2010-2011
NUS Annual Teaching Excellence Award (ATEA) AY2009-2010
NUS Faculty of Science Teaching Excellence Award (FTEA) AY2009-2010
NUS Faculty of Science Teaching Excellence Award (FTEA) AY2008-2009
CDTL (Center for Development of Teaching and Learning) Education Conference Grant Award (AY 2010)
HUGO (Human Genome Organization) Pacific Fellowship Award (2004)

Teaching: Modules/Courses

Research and Communication in Life Sciences (LSM3201): 2013 to Present
Science: The Good, the Bad, the Ugly and the Beautiful (FMS1221B): 2012 to Present
Genes and Society (LSM1302/GEK1527): 2006 to 2016
Fundamentals of Biochemistry (LSM1402): 2006 to 2013
Experimental Molecular and Cell Biology (LSM2202): 2007

Research Interest

  1. Transcriptome, molecular and functional characterization of adaptive response in organs and tissues of fish under specific environmental stressors.

  2. Toxicogenomics, molecular toxicology and toxicity screening applications using the zebrafish model and other organisms.

  3. Characterization of selected channels and transporters of ions and small molecules as potential components of biomimetic membranes used in desalination and water purification.

  4. Enhancing Transferable Skills for Future-ready Graduates

Publications

  1. Li C, Li P, Tan YM, Lam SH, Chan EC, Gong Z.Metabolomic Characterizations of Liver Injury Caused by Acute Arsenic Toxicity in Zebrafish. PLoS One. 2016 Mar 11;11(3):e0151225.

  2. Chen Q, Jia A, Snyder SA, Gong Z, Lam SH. Glucocorticoid activity detected by in vivo zebrafish assay and in vitro glucocorticoid receptor bioassay at environmental relevant concentrations. Chemosphere. 2016 Feb;144:1162-9.

  3. Lam SH, Lui EY, Li Z, Cai S, Sung WK, Mathavan S, Lam TJ, Ip YK. Differential transcriptomic analyses revealed genes and signaling pathways involved in iono-osmoregulation and cellular remodeling in the gills of euryhaline Mozambique tilapia, Oreochromis mossambicus. BMC Genomics. 2014 Oct 23;15:921.

  4. Li Z, Lui EY, Wilson JM, Ip YK, Lin Q, Lam TJ, Lam SH. Expression of key ion transporters in the gill and esophageal-gastrointestinal tract of euryhaline Mozambique tilapia Oreochromis mossambicus acclimated to fresh water, seawater and hypersaline water. PLoS One. 2014 Jan 31;9(1):e87591.

  5. Liu C, Xu H, Lam SH, Gong Z. Selection of reliable biomarkers from PCR array analyses using relative distance computational model: methodology and proof-of-concept study. PLoS One. 2013 Dec 12;8(12):e83954.

  6. 6) Li ZH, Xu H, Zheng W, Lam SH, Gong Z. RNA-sequencing analysis of TCDD-induced responses in zebrafish liver reveals high relatedness to in vivo mammalian models and conserved biological pathways. PLoS One. 2013 Oct 30;8(10):e77292.

  7. Gabor KA, Stevens CR, Pietraszewski MJ, Gould TJ, Shim J, Yoder JA, Lam SH, Gong Z, Hess ST, Kim CH. Super resolution microscopy reveals that caveolin-1 is required for spatial organization of CRFB1 and subsequent antiviral signaling in zebrafish. PLoS One. 2013 Jul 9;8(7):e68759.

  8. Xu H, Lam SH, Shen Y, Gong Z. Genome-wide identification of molecular pathways and biomarkers in response to arsenic exposure in zebrafish liver. PLoS One. 2013 Jul 29;8(7):e68737

  9. Lam SH, Ung CY, Hlaing MM, Hu J, Li ZH, Mathavan S, Gong Z. Molecular insights into 4-nitrophenol-induced hepatotoxicity in zebrafish: transcriptomic, histological and targeted gene expression analyses. Biochim Biophys Acta. 2013 Oct;1830(10):4778-89.

  10. Ching B, Chen XL, Yong JH, Wilson JM, Hiong KC, Sim EW, Wong WP, Lam SH, Chew SF, Ip YK. Increases in apoptosis, caspase activity and expression of p53 and bax, and the transition between two types of mitochondrion-rich cells, in the gills of the climbing perch, Anabas testudineus, during a progressive acclimation from freshwater to seawater. Front Physiol. 2013 Jun 7;4:135.

  11. Ip YK, Soh MM, Chen XL, Ong JL, Chng YR, Ching B, Wong WP, Lam SH, Chew SF. Molecular characterization of branchial aquaporin 1aa and effects of seawater acclimation, emersion or ammonia exposure on its mRNA expression in the gills, gut, kidney and skin of the freshwater climbing perch, Anabas testudineus. PLoS One. 2013 Apr 9;8(4):e61163.

  12. Huang SM, Xu F, Lam SH, Gong Z, Ong CN. Metabolomics of developing zebrafish embryos using gas chromatography- and liquid chromatography-mass spectrometry. Mol Biosyst. 2013 Jun;9(6):1372-80.

  13. Ung CY, Lam SH, Zhang X, Li H, Zhang L, Li B, Gong Z. Inverted expression profiles of sex-biased genes in response to toxicant perturbations and diseases. PLoS One. 2013;8(2):e56668.

  14. Zheng W, Xu H, Lam SH, Luo H, Karuturi RK, Gong Z. Transcriptomic analyses of sexual dimorphism of the zebrafish liver and the effect of sex hormones. PLoS One. 2013;8(1):e53562.

  15. Ge, Y, DZ Wang, JF Chiu, S Cristobal, D Sheehan, F Silvestre, X Peng, H Li, Gong Z, Lam SH, W Hu, H Iwahashi, J Liu, N Mei, L Shi, M Bruno, H Foth and K Teichman, “Environmental OMICS: Current Status and Future Directions.”. Journal of Integrated OMICS, 2013; 3(2): 75-87.

  16. Ng HBG, Lam SH, Sukardi H and Gong Z. Potential applications of transgenic fish to environmental monitoring and toxicology. In Agriculture Biotechnology, eds. Fletcher G L and Rise M L. Oxford: Agriculture Biotechnology, 2012, pp.267-280.

  17. Zhang X, Ung CY, Lam SH, Ma J, Chen YZ, Zhang L, Gong Z, Li B. Toxicogenomic analysis suggests chemical-induced sexual dimorphism in the expression of metabolic genes in zebrafish liver. PLoS One. 2012;7(12):e51971.

  18. Chng HT, Ho HK, Yap CW, Lam SH, Chan EC. An investigation of the bioactivation potential and metabolism profile of Zebrafish versus human. J Biomol Screen. 2012 Aug;17(7):974-86.

  19. Ip YK, Loong AM, Kuah JS, Sim EW, Chen XL, Wong WP, Lam SH, Delgado IL, Wilson JM, Chew SF. Roles of three branchial Na(+)-K(+)-ATPase α-subunit isoforms in freshwater adaptation, seawater acclimation, and active ammonia excretion in Anabas testudineus. Am J Physiol Regul Integr Comp Physiol. 2012 Jul 1;303(1):R112-25.

  20. Ip YK, Wilson JM, Loong AM, Chen XL, Wong WP, Delgado IL, Lam SH, Chew SF. Cystic fibrosis transmembrane conductance regulator in the gills of the climbing perch, Anabas testudineus, is involved in both hypoosmotic regulation during seawater acclimation and active ammonia excretion during ammonia exposure. J Comp Physiol B. 2012 Aug;182(6):793-812.

  21. Sarika J, Ung CY, Bhojwani J, Li B, Zhang L, Lam SH, Gong Z. Spectral analysis of Gene co-expression network of Zebrafish. EPL-Europhysics letters, 2012; 99(4): 410004.

  22. Gong Z, Koh CHV, Nguyen AT, Zhan H, Li Z, Lam SH, Spitsbergen JM, Emelyanov A and Parinov S. The zebrafish model for liver carcinogenesis. In Molecular Genetics of Liver Neoplasia, eds. Xin Wei Wang, Joe W. Grisham and Snorri S. Thorgeirsson. Cancer Genetics, vol. 6, ed. Ostrander, Elaine. New York: Springer, 2011, pp. 197-218.

  23. Lam SH, Hlaing MM, Zhang X, Yan C, Duan Z, Zhu L, Ung CY, Mathavan S, Ong CN, Gong Z. Toxicogenomic and phenotypic analyses of bisphenol-A early-life exposure toxicity in zebrafish. PLoS One. 2011;6(12):e28273.

  24. Loong AM, Chew SF, Wong WP, Lam SH, Ip YK. Both seawater acclimation and environmental ammonia exposure lead to increases in mRNA expression and protein abundance of Na⁺:K⁺:2Cl⁻ cotransporter in the gills of the climbing perch, Anabas testudineus. J Comp Physiol B. 2012 May;182(4):491-506.

  25. Ung CY, Lam SH, Zhang X, Li H, Ma J, Zhang L, Li B, Gong Z. Existence of inverted profile in chemically responsive molecular pathways in the zebrafish liver. PLoS One. 2011;6(11):e27819.

  26. Sukardi H, Zhang X, Lui EY, Ung CY, Mathavan S, Gong Z, Lam SH. Liver X receptor agonist T0901317 induced liver perturbation in zebrafish: histological, gene set enrichment and expression analyses. Biochim Biophys Acta. 2012 Jan;1820(1):33-43.

  27. Nguyen AT, Emelyanov A, Koh CH, Spitsbergen JM, Lam SH, Mathavan S, Parinov S, Gong Z. A high level of liver-specific expression of oncogenic Kras(V12) drives robust liver tumorigenesis in transgenic zebrafish. Dis Model Mech. 2011 Nov;4(6):801-13.

  28. Lam SH, Lee SG, Lin CY, Thomsen JS, Fu PY, Murthy KR, Li H, Govindarajan KR, Nick LC, Bourque G, Gong Z, Lufkin T, Liu ET, Mathavan S. Molecular conservation of estrogen-response associated with cell cycle regulation, hormonal carcinogenesis and cancer in zebrafish and human cancer cell lines. BMC Med Genomics. 2011 May 16;4:41.

  29. Sukardi H, Chng HT, Chan EC, Gong Z, Lam SH. Zebrafish for drug toxicity screening: bridging the in vitro cell-based models and in vivo mammalian models. Expert Opin Drug Metab Toxicol. 2011 May;7(5):579-89.

  30. Lam SH and Gong Z. Fish as a Model for Human Disease. In Vogel and Motulsky ’s Human Genetics – Problem and Approaches, 4 th. edition, eds. M. Speicher, S Antonarakis and A.G. Motulsky. Berlin: Springer-Verlag, 2010. pp. 827-843. [Textbook Chapter]

  31. Wang Z, Du J, Lam SH, Mathavan S, Matsudaira P, Gong Z. Morphological and molecular evidence for functional organization along the rostrocaudal axis of the adult zebrafish intestine. BMC Genomics. 2010 Jun 22;11:392.

  32. Sukardi H, Ung CY, Gong Z, Lam SH. Incorporating zebrafish omics into chemical biology and toxicology. Zebrafish. 2010 Mar;7(1):41-52.

  33. Ung CY, Lam SH, Hlaing MM, Winata CL, Korzh S, Mathavan S, Gong Z. Mercury-induced hepatotoxicity in zebrafish: in vivo mechanistic insights from transcriptome analysis, phenotype anchoring and targeted gene expression validation. BMC Genomics. 2010 Mar 30;11:212.

  34. Duan ZH, Zhu L, Feng MF, Bu WJ, Lam SH and Gong Z. Application of zebrafish microarray on the toxicity mechanism study of bisphenol A. Huan Jing Ke Xue 2010; 31(3):808-814.

  35. Lam SH, Krishna Murthy Karuturi R, Gong Z. Zebrafish spotted-microarray for genome-wide expression profiling experiments: data acquisition and analysis In Zebrafish : Methods and Protocols, Methods in Molecular Biology, vol. 546. Dordrecht. Heidelberg, London, New York: Humana Press, 2009. pp. 197-226.

  36. Lam SH, Mathavan S, Gong Z. Zebrafish spotted-microarray for genome-wide expression profiling experiments. Part I: array printing and hybridization. In Zebrafish: Methods and Protocols. Methods in Molecular Biology, vol. 546. Dordrecht, Heidelberg, London, New York: Humana Press, 2009. pp. 175-195.

  37. Ung CY, Lam SH, Gong Z. Comparative transcriptome analyses revealed conserved biological and transcription factor target modules between the zebrafish and human tumors. Zebrafish. 2009 Dec;6(4):425-31.

  38. Lam SH, Mathavan S, Tong Y, Li H, Karuturi RK, Wu Y, Vega VB, Liu ET, Gong Z. Zebrafish whole-adult-organism chemogenomics for large-scale predictive and discovery chemical biology. PLoS Genet. 2008 Jul 11;4(7):e1000121.

  39. Lam SH, Winata CL, Tong Y, Korzh S, Lim WS, Korzh V, Spitsbergen J, Mathavan S, Miller LD, Liu ET, Gong Z. Transcriptome kinetics of arsenic-induced adaptive response in zebrafish liver. Physiol Genomics. 2006 Nov 27;27(3):351-61.

  40. Lam SH, Gong Z. Modeling liver cancer using zebrafish: a comparative oncogenomics approach. Cell Cycle. 2006 Mar;5(6):573-7.

  41. Lam SH, Wu YL, Vega VB, Miller LD, Spitsbergen J, Tong Y, Zhan H, Govindarajan KR, Lee S, Mathavan S, Murthy KR, Buhler DR, Liu ET, Gong Z. Conservation of gene expression signatures between zebrafish and human liver tumors and tumor progression. Nat Biotechnol. 2006 Jan;24(1):73-5.

  42. Zeng Z, Shan T, Tong Y, Lam SH, Gong Z. Development of estrogen-responsive transgenic medaka for environmental monitoring of endocrine disrupters. Environ Sci Technol. 2005 Nov 15;39(22):9001-8.

  43. Mathavan S, Lee SG, Mak A, Miller LD, Murthy KR, Govindarajan KR, Tong Y, Wu YL, Lam SH, Yang H, Ruan Y, Korzh V, Gong Z, Liu ET, Lufkin T. Transcriptome analysis of zebrafish embryogenesis using microarrays. PLoS Genet. 2005 Aug;1(2):260-76

  44. Lam SH, Sin YM, Gong Z, Lam TJ. Effects of thyroid hormone on the development of immune system in zebrafish. Gen Comp Endocrinol. 2005 Jul;142(3):325-35.

  45. Lam SH, S Mathavan, Y Tong, J Hu, C L Winata, S Lee, L D Miller, E T Liu and Z Y Gong. Preliminary microarray analyses of gene expression in zebrafish treated with xenobiotic and bioactive compounds. Marine Biotechnology. 2005; 6:S468-474.

  46. Tong Y, Shan T, Poh YK, Yan T, Wang H, Lam SH, Gong Z. Molecular cloning of zebrafish and medaka vitellogenin genes and comparison of their expression in response to 17beta-estradiol. Gene. 2004 Mar 17;328:25-36.

  47. Lam SH, Chua HL, Gong Z, Lam TJ, Sin YM. Development and maturation of the immune system in zebrafish, Danio rerio: a gene expression profiling, in situ hybridization and immunological study. Dev Comp Immunol. 2004 Jan;28(1):9-28.

  48. Lam SH, Chua HL, Gong Z, Wen Z, Lam TJ, Sin YM. Morphologic transformation of the thymus in developing zebrafish. Dev Dyn. 2002 Sep;225(1):87-94.