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Teaching Areas

General Virology (LSM3227)

Laboratory Techniques in Life Sciences (LSM2191)

Biological Challenges & Opportunities for Humankind (LSM1111)

Research Areas

Virology, Aquaculture, Aquatic Viruses and Viral Diseases, Shrimp White Spot Syndrome Virus (WSSV), Fish Iridoviruses, SGIV

Research Interests

I have an avid interest in aquaculture and aquatic viral diseases, while focus on two large DNA viruses: white spot syndrome virus (WSSV) and Singapore grouper iridovirus (SGIV). Through collaboration with experts in proteomics, X-ray crystallography, NMR spectroscopy and cryo-electron microscopy, we are able to study virus-host interactions, solve the structures of both viral proteins and viral particles. Our findings not only assist in the development of antiviral strategies drugs but also serve as a paradigm for understanding viral morphogenesis.

Publications in Education

1. Wu, J., Van Nguyen, H., Chen, R., Fan, P. & Loh, K. (2017). The combined effects of physical and virtual models in learning cellular biology. In H. Partridge, K. Davis, & J. Thomas. (Eds.), Me, Us, IT! Proceedings ASCILITE2017: 34th International Conference on Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education (pp. 168 -172). (corresponding)

2. Wu J. (2016) Learning through Video Production – an Instructional Strategy for Promoting Active Learning in a Biological Course. In S. Barker, S. Dawson, A. Pardo, & C. Colvin (Eds.), Show Me The Learning. Proceedings ASCILITE 2016 Adelaide (pp. 657-662).

3. Wu J. (2013) Mutation-Based Learning to Improve Student Autonomy and Scientific Inquiry Skills in a Large Genetics Laboratory Course. CBE Life Sci. Educ. 12:460-470.

4. Wu J and Ho D (2011) Student-designed mutations on experimental protocols: Promoting students’ ownership of learning in a teaching laboratory. Teaching & Learning in Higher Education (Dec 6-9), conference paper

5. Wu J. (2009) Linking Assessment Questions to a Research Article to Stimulate Self-directed Learning and Develop High-order Cognitive Skills in an Undergraduate Module of Molecular Genetics. CBE Life Sci. Educ. 8:283-290. (selected in Highlights)

6. Wu J (2008) Assessing Students’ Learning Using Questions Linked with a Research Article. Teaching & Learning in Higher Education (Dec 3-5), TLHE conference proceedings (2008)

Publications in Research

1. Tan X., Ravasio A., Ong HT, Wu J., Hew CL (2020) White spot syndrome viral protein VP9 alters the cellular higher‐order chromatin structure, FASEB BioAdvances, https://doi.org/10.1096/fba.2019-00086 (co-corresponding author)

2. Pintilie G, Chen DH, Tran BN, Jakana J, Wu J, Hew CL, Chiu Wah (2019) Segmentation and Comparative modeling in an 8.6 Å Cryo-EM map of the Singapore Grouper Iridovirus, Structure 27(10)

3. Liu Y, Tran BN, Wang F, Ounjai P, Wu J, Hew CL. (2016) Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells. Sci. Rep. 5, 18696; doi: 10.1038/srep18696 (co-corresponding author)

4. Wang F, Liu Y, Zhu Y, Tran BN, Wu J, Hew CL. (2015) Singapore Grouper Iridovirus ORF75R is a Scaffold Protein Essential for Viral Assembly. Sci. Rep. 5, 13151; doi: 10.1038/srep13151 (co-corresponding author)

5. Wang L, Qiong QY, Wu J. (2013) A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus. Virus Res. 176:37-44. (corresponding author)

6. Tran BN, Chen L, Liu Y, Wu J, Velázquez Campoy A, Sivaraman J, Hew CL. (2011) A Novel Histone H3 Binding Protein ORF158L from the Singapore Grouper Iridovirus (SGIV). J Virol. 85(17): 9159–9166.

7. Liu Y., Wu J., Chen H., Hew CL, Yan J. (2010) DNA Condensates Organized by the Capsid Protein VP15 in White Spot Syndrome Virus. Virology 408:197-203. (co-first author)

8. Wu J, Chan R, Wenk MR, Hew CL (2010) Lipidomic study of intracellular Singapore grouper iridovirus (SGIV). Virology 399: 248-56.

9. Wu J, Lin Q, Lim TK, Liu T, Hew CL (2007) White spot syndrome virus proteins and differentially expressed cellular proteins identified in shrimp epithelium by shotgun proteomics and cleavable isotope-coded affinity tag. J. Virol. 81:11681-9.

10. Li Z, Lin Q, Chen J. Wu JL, Lim TK, Loh SS, Tang X, Hew CL (2007) Shotgun identification of Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes. Mol. Cell. Proteomic. 6:1609-20.

11. Leu JH, Chang CC, Wu JL, Hsu CW, Hirono I, Aoki T, Juan HF, Lo CF, Kou GH, Huang HC (2007)Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics, 8:120.

12. Tang X, Wu J, Sivaraman J and Hew CL (2007) Crystal Structure of Two Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus (WSSV) Shed Light on their Evolutionary Relationship. J. Virol. 81:6709–6717.

13. Liu Y, Wu J, Song JX, Sivaraman J, Hew CL (2006) Identification of a novel nonstructural protein VP9 from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites. J. Virol. 80:10419-27.

14. Lo, CF, Wu JL, Chang YS, Wang HC, Tsai JM, Kou GH (2004) Molecular characterization and pathogenicity of white spot syndrome virus. In: Current Trends in Bacterial & Viral Fish & Shrimp Diseases

15. Wu JL, Muroga K (2004) Apoptosis does not play an important role in the resistance of ‘immune’ Penaeus japonicus against white spot syndrome virus. J. Fish Dis. 27:15-21.

16. Namikoshi A, Wu JL, Yamashita T, Nishizawa T, Nishioka T, Arimoto M, Muroga K (2004) Vaccination trials in Penaeus japonicus against white spot syndrome virus. Aquaculture 229:25-35.

17. Wu JL, Nishioka T, Mori K, Nishizawa T, Muroga K (2002) A time-course study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol. 13:391-403.

18. Wu JL, Suzuki K, Arimoto M, Nishizawa T, Muroga K (2002) Preparation of an inoculum of white spot syndrome virus for challenge tests in Penaeus japonicus. Fish Pathol. 37:65-70.

19. Wu JL, Namikoshi A, Nishizawa T, Mushiake K, Teruya K, Muroga K (2001) Effects of shrimp density on transmission of penaeid acute viremia in Penaeus japonicus by cannibalism and the waterborne route. Dis. Aquat. Org. 47:129-135.