{"id":4926,"date":"2020-10-05T16:36:10","date_gmt":"2020-10-05T08:36:10","guid":{"rendered":"https:\/\/www.dbs.nus.edu.sg\/?p=4926"},"modified":"2024-08-22T19:01:05","modified_gmt":"2024-08-22T11:01:05","slug":"cbis-confocal-3i-dispim","status":"publish","type":"post","link":"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/","title":{"rendered":"CBIS LM Core: 3I diSPIM"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"4926\" class=\"elementor elementor-4926\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c067336 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c067336\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-b1e1244\" data-id=\"b1e1244\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b6c85b2 elementor-widget elementor-widget-image\" data-id=\"b6c85b2\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"640\" height=\"480\" src=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-1024x768.jpg\" class=\"attachment-large size-large wp-image-1644\" alt=\"\" srcset=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-1024x768.jpg 1024w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-300x225.jpg 300w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-768x576.jpg 768w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-1536x1152.jpg 1536w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-2048x1536.jpg 2048w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-46eb306\" data-id=\"46eb306\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-af42900 elementor-widget elementor-widget-text-editor\" data-id=\"af42900\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h2 style=\"margin-bottom: 0px; padding-bottom: 0px;\">3i diSPIM<\/h2>\n<h5 style=\"margin-top: 0px; padding-top: 0px; text-transform: uppercase;\">Marianas LightSheet<\/h5>\n<p>Live imaging directly involves inevitable tradeoffs of spatial resolution, temporal resolution and phototoxicity. This is especially true when interrogating imaging in three dimensions and\/or three-dimensional samples over time (3D and\/or 4D imaging) to obtain a complete picture of many dynamic subcellular processes. Selective Plane Illumination Microscopy (or Light Sheet Microscopy) uses a separate excitation lens perpendicular to the widefield detection lens to illuminate only the plane of interest within sheet of light. Its low photo toxicity, photo bleaching, high signal-to-noise ratio and high imaging speed make it a favourite tool for live imaging.<\/p>\n<p><strong>Location<\/strong><span>:\u00a0<\/span><a href=\"https:\/\/www.dbs.nus.edu.sg\/cbis\/cbis-services\/confocal-microscopy-laboratory\/\" target=\"_blank\" rel=\"noopener\">CBIS Light Microscopy Core<\/a> (S1A #01-04 CBIS lab)<br \/><strong>Booked CBIS equipment before?<\/strong><span>\u00a0<\/span><a href=\"https:\/\/www.dbs.nus.edu.sg\/cbis\/cbis-services\/cryoelectron-microscopy-facility\/cbis-confocal-first-time-users\/\" target=\"_blank\" rel=\"noopener\">Information for First Time Users<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-995616d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"995616d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-235e7ff\" data-id=\"235e7ff\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f1ab783 elementor-widget elementor-widget-button\" data-id=\"f1ab783\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.dbs.nus.edu.sg\/cbis\/cbis-services\/confocal-microscopy-laboratory\/cbis-confocal-first-time-users#forms\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Application<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-59515e8\" data-id=\"59515e8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7ba0bf1 elementor-widget elementor-widget-button\" data-id=\"7ba0bf1\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/www.dbs.nus.edu.sg\/cbis\/cbis-services\/confocal-microscopy-laboratory\/cbis-confocal-policies-fees\/\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Policies\/Fees<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-8a19fe6\" data-id=\"8a19fe6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-aa13128 elementor-widget elementor-widget-button\" data-id=\"aa13128\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/ppms.asia\/singascope\/login\/?pf=7\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Booking (VPN)<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6764a4a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6764a4a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c75c84e\" data-id=\"c75c84e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-30e90d2 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"30e90d2\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0944ebd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0944ebd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-04013e8\" data-id=\"04013e8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-945f695 elementor-widget elementor-widget-text-editor\" data-id=\"945f695\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>About Marianas LightSheet<\/h4>\n<p>Marianas LightSheet\u2122 from Intelligent Imaging Innovations, Inc. (3I, USA) merges dual inverted selective plane illumination (diSPIM) with the power and flexibility of a live-cell microscopy system. It employs two orthogonal objectives positioned at 45\u00b0 above the specimen plane. By alternating between the objectives for imaging and excitation, diSPIM captures two volumes that are fused and deconvolved to achieve isotropic resolution. Unlike capillary-based light sheet methods, diSPIM allows for standard specimen preparation in standard dishes and standard media.<\/p>\n<h4><img decoding=\"async\" width=\"575\" height=\"381\" src=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/2018-3i-diSPIM-interior-Picture1.png\" alt=\"\" class=\"alignnone size-full wp-image-1645\" srcset=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/2018-3i-diSPIM-interior-Picture1.png 575w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/2018-3i-diSPIM-interior-Picture1-300x199.png 300w\" sizes=\"(max-width: 575px) 100vw, 575px\" \/><\/h4>\n<p>The 3I diSPIM at CBIS\/DBS confocal core, offers a single- (iSPIM) or dual- (diSPIM) sided light-sheet arrangement on an inverted microscope with custom built OKO environment chamber for live cell imaging and photon manipulation unit for FRAP. 3D isotropic resolution of 360 nm in x-y-z is achievable (by beads). Samples are prepared on conventional glass coverslips and agarose gel for monolayer cell culture and small organisms imaging respectively.<\/p>\n<p><img decoding=\"async\" width=\"258\" height=\"258\" src=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/unfused_3D-1.tif-RGB.jpg\" alt=\"\" class=\"aligncenter size-full wp-image-26720\" srcset=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/unfused_3D-1.tif-RGB.jpg 258w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/unfused_3D-1.tif-RGB-150x150.jpg 150w\" sizes=\"(max-width: 258px) 100vw, 258px\" \/> <img loading=\"lazy\" decoding=\"async\" width=\"258\" height=\"258\" src=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/fused_3D-1.tif-RGB.jpg\" alt=\"\" class=\"size-full wp-image-26721 aligncenter\" srcset=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/fused_3D-1.tif-RGB.jpg 258w, https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/fused_3D-1.tif-RGB-150x150.jpg 150w\" sizes=\"(max-width: 258px) 100vw, 258px\" \/><\/p>\n<p>Top, single-sided light sheet; bottom, diSPIM with isotropic resolution plus deconvolution. U2OS cell with CENP-A and tubulin. Sample: Zemin Jiang, Assoc Prof Yih-Cherng Liou.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-3880b66\" data-id=\"3880b66\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c732b8a elementor-widget elementor-widget-text-editor\" data-id=\"c732b8a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h5>Help<\/h5>\n<ul>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-CBIS-diSPIM_short-manual.pdf\" target=\"_blank\" rel=\"noopener\">Quick Reference<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-Marianas-LightSheet-SlideBook-Manual.pdf\" target=\"_blank\" rel=\"noopener\">Manual<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/07\/A-guide-to-mounting-zebrafish-larvae-for-diSPIM-imaging.pdf\" target=\"_blank\" rel=\"noopener\">Live zebrafish sample preparation protocol (Dr Cathleen Teh)<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/08\/diSPIM-cell-sample-mounting-protocol.pdf\" target=\"_blank\" rel=\"noopener\">Live cell sample preparation and mounting protocol<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/07\/3i-diSPIM-recon_decon.pdf\" target=\"_blank\" rel=\"noopener\">Multiview reconstruction (Slidebook)<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/07\/3i-diSPIM-data-Reconstruction-with-Fiji.pdf\" target=\"_blank\" rel=\"noopener\">Multiview reconstruction (Fiji)<\/a><\/li>\n<li><a href=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2024\/07\/diSPIM-deconvolution-in-Huygens.pdf\" target=\"_blank\" rel=\"noopener\">Deconvolution (Huygens)<\/a><\/li>\n<\/ul>\n<h5><\/h5>\n<h5>Features<\/h5>\n<ul>\n<li>Modalities: Single and dual inverted SPIM (iSPIM and diSPIM)<\/li>\n<li>iSPIM x-y-z resolution: 360 x 360 x 760 nm<\/li>\n<li>diSPIM x-y-z resolution: 360 x 360 x 360 nm<\/li>\n<li>SPIM objectives: Nikon water dipping objectives: CFI NIR Apo 40X\/0.8 NA WD 3.5 mm, x2; CFI Plan Fluor 10x\/0.3NA WD 3.5mm, x2.<\/li>\n<li>Finder objectives: Zeiss 40x and 10x<\/li>\n<li>Sample platform: Rectangular coverslips (1.5#, 24 x 50 mm) or agarose on petri dishes<\/li>\n<li>Excitation sources: 405nm (100mW), 488nm (50mW), 561nm (50mW) and 640 nm (100mW)<\/li>\n<li>Dichroic mirror: Shemrock410\/504\/582\/669 nm BrightLine\u00ae quad-edge dichroic beamsplitter<\/li>\n<li>Emission filters Shemrock440\/521\/607\/700 nm BrightLine\u00ae quad-band bandpass filter<\/li>\n<li>Light-sheet camera: Twin Hamamatsu ORCA-Flash4.0 V3 sCMOS<\/li>\n<li>Environmental control: diSPIM custom designed OKO incubator with temperature, CO2 and humidity control<\/li>\n<li>Software: Slidebook 6<\/li>\n<li>Operating system: Windows 10<\/li>\n<li>Data transfer: 10 GB network connection \u2013 no external hard drives allowed<\/li>\n<li>Usage: Staff-assisted and self-use allowed (following training)<\/li>\n<\/ul>\n<h5><\/h5>\n<h5>Applications<\/h5>\n<ul>\n<li>3D-5D Live cell imaging: multi-channel, 3D and time lapse imaging for isotropic resolution<\/li>\n<li>3D-5D Live small organism imaging<\/li>\n<li>Advanced F-techniques: FRAP<\/li>\n<\/ul>\n<h5><\/h5>\n<h5>Software<\/h5>\n<ul>\n<li>3i Slidebook<\/li>\n<\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Live imaging directly involves inevitable tradeoffs of spatial resolution, temporal resolution and phototoxicity. This is especially true when interrogating imaging in three dimensions and\/or three-dimensional samples over time (3D and\/or 4D imaging) to obtain a complete picture of many dynamic subcellular processes.<\/p>\n","protected":false},"author":98,"featured_media":1644,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[105],"tags":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>CBIS LM Core: 3I diSPIM - NUS Biological Sciences<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CBIS LM Core: 3I diSPIM - NUS Biological Sciences\" \/>\n<meta property=\"og:description\" content=\"Live imaging directly involves inevitable tradeoffs of spatial resolution, temporal resolution and phototoxicity. This is especially true when interrogating imaging in three dimensions and\/or three-dimensional samples over time (3D and\/or 4D imaging) to obtain a complete picture of many dynamic subcellular processes.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\" \/>\n<meta property=\"og:site_name\" content=\"NUS Biological Sciences\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/NUSbiologicalsciences\/\" \/>\n<meta property=\"article:published_time\" content=\"2020-10-05T08:36:10+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-08-22T11:01:05+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-scaled.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1920\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"deedupuy\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"deedupuy\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\"},\"author\":{\"name\":\"deedupuy\",\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/#\/schema\/person\/1e57cbb64bcdff15eaa6cbe6a070a9d8\"},\"headline\":\"CBIS LM Core: 3I diSPIM\",\"datePublished\":\"2020-10-05T08:36:10+00:00\",\"dateModified\":\"2024-08-22T11:01:05+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\"},\"wordCount\":484,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/#organization\"},\"image\":{\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.dbs.nus.edu.sg\/wp-content\/uploads\/sites\/7\/2020\/03\/diSPIM-picture-scaled.jpg\",\"articleSection\":[\"CBIS Light Microscopy Core\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\",\"url\":\"https:\/\/www.dbs.nus.edu.sg\/2020\/10\/05\/cbis-confocal-3i-dispim\/\",\"name\":\"CBIS LM Core: 3I diSPIM - 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