Wong's Molecular Virology Lab

 

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People

 

Principal Investigator

Sek-Man Wong
Professor
Department of Biological Sciences,
National University of Singapore
E-mail: dbswsm

Curriculum vitae

 

Graduate Students

Years
Name
Project title
2005-
Cheng Ao
Application of HCRSV as nano-materials.
2006-
Zhang Xin
Interaction of HCRSV with kenaf.
2006-
Sunil Kumar Tewary
Structural study of HLSV.
2007-
Xie Juntao
Analysis of protein profiles of Arabidopsis infected with HCRSV and TCV using 2-D gel electrophoresis.


Alumni

PhD students ( As major supervisor:)

Years

Name

Project Title

1996-1999

Yu Haihui

Molecular manipulation of two orchid viruses.

1996-2000

Lee Kian Chung

Molecular analysis of the Singapore isolate of zucchini yellow mosaic virus.
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1997-2000

Li Hongwei

Roles of Tomato Aspermy Virus 2b in plant hypersensitive resistance and post transcriptional gene silencing defense.

1998-2002

Liang Xiaozhen

Molecular characterization of the P23 and coat protein of Hibiscus chlorotic ringspot virus.

1999-2003

Koh Chin Yen

Studies on translational mechanisms of Hibiscus chlorotic ringspot virus (HCRSV).

1998-2003

Prabha Aruna Ajjikuttira

Genetic variability and interactions of cymbidium mosaic virus and odontoglossum ringspot virus.

1998-2003

Kandhadayar Gopalan Srinivasan

Molecular characterization of a novel Tobamovirus infecting hibiscus.

2002-2005

Meng Chunying

Mechanism of gene silencing suppression by Hibiscus chlorotic ringspot virus coat protein.

2002-2006

Wang Xiaoxing

Expression of Hepatitis C virus capsid protein using a plant viral vector.

2003-2006

Cao Shishu

Functional analysis of an internal polyA tract in Hibiscus latent Singapore virus.


PhD students (as co-supervisors):

1995-1999

Leina Joseph

Host pathogen interaction in pseudocercospora leaf spot disease.

1997-2000

Chen Xiaole

Molecular study of vandaceous orchids using amplified fragment length polymorphism.

1997-2000

Theingi Maw

Production of chitosan and isolation of the chitin deacetylase gene from Gongronalia butleri (Leander) Peyronel and Dal Vesco.

MSc degree ( As major supervisor):

 

 

 

1989-1992

Lee Sor Cheng

Molecular studies of zucchini yellow mosaic virus.

1989-1992

Neo Koh Koh

Molecular cloning and study of the RNA genome of cymbidium mosaic virus.

1991-1993

Lim Siew Tin

Production of virus - free orchids via tissue culture techniques.

1993-1995

Tan Hui Ning, Priscilla

Molecular cloning and characterization of Singapore ageratum yellow vein virus.

1993-1995

Tan Yan

Molecular analysis of cymbidium mosaic virus.

1992-1996

Weng Lijuan

Genetic analysis hibiscus chlorotic ringspot virus.

1992-1996

Chng Chee Giok

The complete genomic structure of a Singapore isolate of Odontoglossum Ringspot Tobamovirus.

1995-1997

Hu Wenwei

Synergism in replication of cymbidium Mosaic potexvirus and odontoglossum Ringspot Tobamovirus RNA in orchid protoplast.

1997-1998

Seoh Mui Leng

Quantification and simultaneous RT-PCR detection of cymbidium Mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) in orchid tissue.

1997-1998

Sharon Thio

Site-directed mutagenesis at the coat protein green of cucumber mosaic virus (CMV) involving movement in squash.

1997-1999

Koh Li Huan

Molecular cloning of Broad Bean Wilt Fabavirus from Megakespama erythrochlamys.

1998-2000

Stella Tan

Glycosylation and acetylation of coat proteins of plant viruses.

1998-2000

Yvonne Soh

Investigation of viral movement in plants.

1998-2000

Aileen Lim

Expression of Nocistatin using a plant viral vector.

1998-2000

Tricia Otega

Genetic manipulation of the RdRp and movement protein of Tobamovirus.

1998-2000

Lim Jade Hsia

Viral vector expression of mite allergens.

1998-2000

Alvin Eun

Application of molecular beacons and quartz crystal microbalance immunosensors for the detection of two orchid viruses.

2003-2005

Luo Qiong

Microarray analyses of HCRSV and TCV infected Arabidopsis thaliana.

 

MSc degree (As Co-supervisor)

 

1992-1995

Shang Huisheng

Molecular studies PET 61, PET44 and YKE2 genes. in Saccharomtces cerevisiae.

1995-1997

Tan Su Ching

A study on the isolation and characterization of fungal chitosan.

1998-2000

Ma Ming

Evaluation of Rhizoctonia fungi using morphological, molecular and fungal-plant interaction approaches.

2004-2006

Chai Xiujuan, Davina

Production of virus-resistant orchids.

2004- 2006

Chen Hongyan

Identification of SOC1 target genes in flowering process.

 

Honours degree ( As major supervisor):

Years

Name

Project Title

 

 

 

1988-1989

Francis Soo

Survey of cymbidium mosaic and odontoglossum ringspot virus and characterization of cymbidium mosaic virus.

1988-1989

Lee Sor Cheng

Investigation of viruses infecting cucumbers (Cucumis sativus L.) grown in a hydroponic farm and isolation of viral double?stranded ribonucleic acids.

1989-1990

Chng Chee Yeong

Isolation, purification and characterisation of a Singapore isolate of zucchini yellow mosaic virus and its inclusion bodies for infested leaf tissues of Cucurbita species.

1989-1990

Lim Soo Hwee

Isolation, purification and serological studies on 3 isolates of odontoglossum ringspot virus for Cattleya and Spathoglottis species in Singapore.

1991-1992

Pamela Ang

Studies of some physico-chemical properties of odontoglossum ringspot virus.

1991-1992

Tan Hui Ning, Priscilla

Characterisation of a germinivirus in Ageratum conyzoides.

1992-1993

Cheong Oi Lin, Angeline

Studies on the overexpression of two putative movement protein genes of CymMV and their subcellular localization in CymMV - infected orchid tissue.

1992-1993

Yvonne Chan Leng Leng

Molecular cloning of the replicase gene of cymbidium mosaic virus.

1992-1993

Pua Chieh Sze

Molecular analysis of the small nuclear inclusion protein gene of zucchini yellow mosaic virus.

1992-1993

Tan Ching Ting

Molecular cloning of the polymerase gene of zucchini yellow mosaic virus towards producing transgenic plants.

1993-1994

Tracy Gan

Characterization of Broad bean wilt virus from Megakepasma erythrochlamys L.

1995-1996

Lee Kian Chung

Complete nucleotide sequence determination and phylogenetic analysis of cytoplasmic inclusion protein gene of zucchini yellow mosaic virus.

1995-1996

Mahtani Parvesh Hariram

Molecular characterization phylogenetic analysis of RNA-dependant RNA polymerase (RdRp) of odontoglossum ringspot virus (ORSV).

1995-1996

Caroline Ho Lee Ling

Replication of Cymbidium mosaic virus and odontoglossum ringspot virus in orchid protoplasts and their detections using non-radioactive RNA porbes.

1996-1997

Chang Min Li

Molecular characterization of Hibiscus chlorotic ringspot virus (HCRSV).

1996-1997

Koh Li Huan

Investigation of a mosaic disease of viral etiology from Bougainvillea.

1996-1997

Suryah Bte Mohamad

Cross protection phenomenon between TMV and ORSV in nicotiana benthamiana and replication kinetics of TMV and ORSV in protoplasts.

1996-1997

Seoh Mui Leng

Simultaneous PCR detection of cymbidium mosaic virus (CymMV) and odontoglossum ringspot virus (ORSV) in orchid tissue.

1997-1998

Sharon Thio Swee Chin

Site-directed mutagenesis at the coat protein green of cucumber mosaic virus (CMV) involving movement in squash.

1997-1998

Stella Tan Wei Ling

Investigation of coat protein glycosylation of cymbidium mosaic potexvirus and odontodoglossum ringspot tobamovirus.

1997-1998

Alvin Eun

Detection of CYMV & ORSV using immuno-capture heads and capillary zone electrophoresis.

1997-1998

Lisa Ng

Characterization of a novel cucumovirus isolated from periwinkle in Singapore.

1997-1998

Joy Quek

Using tobacco protoplasts to study replication of Odontoglossum rinspot and Tobacco mosaic Tobamovirus.

1997-1998

Lim Mei Thorng

Isolation of squash protoplasts and accumulation of coat protein of zucchini yellow mosaic potyvirus.

1998-1999

Koh Chin Yen

Molecular characterization of hibiscus chlorotic ringspot carmovirus.

1998-1999

Zuzy Anggriany Abd Hamid

Cross protection of tobacco mosaic virus in odontoglossum ringspot virus protected Nicotiana benthamiana L.

1999-2000

Roger Chua Peng Jin

Towards synthesis of a biologically active cDNA of zucchini yellow mosaic virus - Singapore isolate.

2001-2002

Hema D/O Jethanand

Detection of orchid viruses using micro-PCR and capillary electrophoresis.

2001-2002

Goh Hwee Kwan

Protein expression of a novel open reading frame (p25) from Hibiscus chlorotic ringspot virus.

2001-2002

Leslie Toh Pee Li

Investigation of the etiology of yellow decline of the rain tree in Singapore.

2003-2004

Tan Teng Ee

Production of monoclonal antibodies against Hibiscus chlorotic ringspot virus.

2004-2005

Tew Wooi Chuan

Expression of SARS virus N protein using Picchia pastoris.

2004-2005

Fok Yu Ting

Production of monoclonal antibodies against SARS virus N protein.

2005-2006

Lai Yiyang

Construction of Cymbidium mosaic virus as a vector for expression of HCV core protein.

2005-2006

Loo Soon Guan

Generation of a biologically active cDNA clone of Zucchini yellow mosaic virus.

2005-2006

Chia Shimin Annabelle

Development of Hibiscus chlorotic ringspot virus into a viral vector.

 

Honours degree (As co-supervisor):

2002-2003

Melissa Sim

Structure-function analysis of mia 1 p a new microtube-binding protein from fission yeast.

2003-2004

Chen Zhen Cheng, Clarice

Viral detection by the NASBA method.

University Research Opportunity Project in Science/Final Year Research Project Students:

2005-2006 Bak Xiao Ying

2005-2006 Pang Junxiong Vincent

2004-2005 Lai Yi Yang

2003-2004 Soo Woon Siew

2003-2004 Chung Jinwei

2003-2004 Cheng Khor Xin

2003-2004 Valerie Teo

2003-2004 Dai Liang

2003-2004 Lim Chin Sim, Eunice

2003-2004 Arthur Luhur

2003-2204 Choah Wee Su

2003-2004 Ho Peiying

2003-2004 Clement Cheong Yuh Meng

2001-2002 Daina Lim

2000-2001 Evinna Low Lin Hui

2000-2001 Goh Hwee Kwan

2000-2001 Lau Pei San

2000-2001 Melissa Sim Yunn Tyug

1999-2000 Yeo Chow Khoon

1998-1999 Mohamed Shali

1998-1999 Cherry Ong Ying Ying

1998-1999 Evonne Low

1998-1999 Emily Lim

1998-1999 Rubina Pasha

1998-1999 Koh Bee Fong

1997-1998 Eiseli Loh Wan Choo

1997-1998 Md. Khairil

1997-1999 Leong Chik Kee

1996-1997 Tan May May

1996-1997 Jennifer Kee Peck Wai


Student Assistant:

2004 Fok Yu Ting

2003 Low Hong San

 

Research Assistant:

2004-2007 Lim Chin Chin

2004-2005 Choah Wee Su

2003-2004 Srinivasan KG

1997-1998 Yap Yun Kiam

 

Research Fellow:

2006-2007 Meng Chunying

2004-2006 Li Weimin

2003-2004 Wang Haihe

2000-2002 Lee Kian Chung

 

National Science & Technology Board Post-Doctoral Fellow:

2001-2002 Wang Haihe

 

Visiting Scientist:

2003-2004 Ms Liao Yongmei ( Guangxi University, PRC)

 

Last updated: 27-Jan-2007