FCS requires a small observation volume to fluorescence intensity fluctuations caused by single
molecules. In confocal FCS this is provided by the laser focus and the confocal pinhole. In camera
based imaging FCS the limitation of the observation volume is achieved by the finite extent of the
pixels (or group of pixels) on the camera chip and a laser illumination that provides intrinsic
z-sectioning. The z-sectioning is achieved either by a thin light sheet generated by a Selective
Plain Illumination Microscope (SPIM)
or the exponentially decaying evanescent wave generated due to Total Internal Reflection (TIR). This
CDF
file aims to provide some demonstrations for Imaging FCS and how their parameters change with
the properties of the system. The Wolfram CDF player needs to be installed to view CDF files. The
CDF player can be downloaded
here.
